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Directed Evolution and Predictive Modelling of Galactose Oxidase towards Bulky Benzylic and Unactivated Secondary Alcohols

Wan Lin Yeo, Dillon W. P. Tay, Jhoann M.T. Miyajima, Shreyas Supekar, Tong Mei Teh, Jin Xu, Yee Ling Tan, Jie Yang See, Hao Fan, Sebastian Maurer-Stroh, Yee Hwee Lim, Ee Lui Ang

Version 1 posted 17 February 2023

447

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Catalysis

Working Paper

Directed Evolution of Flavin-Dependent Halogenases for Atroposelective Halogenation of 3-Aryl-4(3H)-quinazolinones via Kinetic or Dynamic Kinetic Resolution

Harrison M. Snodgrass, Dibyendu Mondal, Jared Lewis

Version 1 posted 26 April 2022

504

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Biological and Medicinal Chemistry

Working Paper

An Engineered Nonribosomal Peptide Synthetase Shows Opposite Amino Acid Loading and Condensation Specificity

Aleksa Stanišić, Annika Hüsken, Philipp Stephan, David L. Niquille, Jochen Reinstein, Hajo Kries

Version 1 posted 16 March 2021

841

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Catalysis

Working Paper

Biocatalytic Conversion of 5-Hydroxymethylfurfural by Galactose Oxidase: Toward Scalable Technology Using Integrated Process and Enzyme Engineering

William Birmingham, Asbjørn Toftgaard Pedersen, Mafalda Dias Gomes, Mathias Bøje Madsen, Michael Breuer, John Woodley, Nicholas J. Turner

Version 1 posted 08 September 2020

562

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2

Citations

Materials Science

Working Paper

Developing a SARS-CoV-2 Antigen Test Using Engineered Affinity Proteins

Seunghyeon Kim, Emma H. Yee, Eric A. Miller, Yining Hao, Dousabel M. Y. Tay, Ki-Joo Sung, Huan Jia, Joseph M. Johnson, Mohsan Saeed, Charles R. Mace, Deniz Yüksel Yurt, Hadley Sikes

Version 1 posted 19 April 2021

757

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Catalysis

Working Paper

In Vivo Assembly of Artificial Metalloenzymes and Application in Whole‐Cell Biocatalysis

Shreyans Chordia, Siddarth Narasimhan, Alessandra Lucini Paioni, Marc Baldus, Gerard Roelfes

Version 1 posted 17 June 2020

771

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Physical Chemistry

Working Paper

Directed evolution of a bright variant of mCherry: Suppression of non-radiative decay by fluorescence lifetime selections

Srijit Mukherjee, Premashis Manna, Sheng-Ting Hung, Felix Vietmeyer, Pia Friis, Amy Palmer, Ralph Jimenez

Version 1 posted 16 March 2022

1,414

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1

Citations

Catalysis

Working Paper

Analysis of Laboratory-Evolved Flavin-Dependent Halogenases Affords a Computational Model for Predicting Halogenase Site Selectivity

Mary C. Andorfer, Declan Evans, Song Yang, Cyndi Q. He, Anna M. Girlich, Jaylie Vergara-Coll, Narayanasami Sukumar, K. N. Houk, Jared C. Lewis

Version 1 posted 16 February 2022

423

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Catalysis

Working Paper

A Designed Photoenzyme Promotes Enantioselective [2+2]-Cycloadditions via Triplet Energy Transfer

Jonathan S. Trimble, Rebecca Crawshaw, Florence J. Hardy, Colin W. Levy, Murray J.B. Brown, Douglas E. Fuerst, Derren Heyes, Richard Obexer, Anthony P. Green

Version 1 posted 13 May 2022

1,650

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Catalysis

Working Paper

Directed Evolution of an Feᴵᴵ-Dependent Halogenase for Asymmetric C(sp³)-H Chlorination

Sabine Duewel, Luca Schmermund, Tabea Faber, Klaus Harms, Vasundara Srinivasan, Eric Meggers, Sabrina Hoebenreich

Version 1 posted 05 November 2019

846

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40 results in Directed Evolution in Keywords: Directed Evolution

Directed Evolution and Predictive Modelling of Galactose Oxidase towards Bulky Benzylic and Unactivated Secondary Alcohols

Directed Evolution of Flavin-Dependent Halogenases for Atroposelective Halogenation of 3-Aryl-4(3H)-quinazolinones via Kinetic or Dynamic Kinetic Resolution

An Engineered Nonribosomal Peptide Synthetase Shows Opposite Amino Acid Loading and Condensation Specificity

Biocatalytic Conversion of 5-Hydroxymethylfurfural by Galactose Oxidase: Toward Scalable Technology Using Integrated Process and Enzyme Engineering

Developing a SARS-CoV-2 Antigen Test Using Engineered Affinity Proteins

In Vivo Assembly of Artificial Metalloenzymes and Application in Whole‐Cell Biocatalysis

Directed evolution of a bright variant of mCherry: Suppression of non-radiative decay by fluorescence lifetime selections

Analysis of Laboratory-Evolved Flavin-Dependent Halogenases Affords a Computational Model for Predicting Halogenase Site Selectivity

A Designed Photoenzyme Promotes Enantioselective [2+2]-Cycloadditions via Triplet Energy Transfer

Directed Evolution of an Feᴵᴵ-Dependent Halogenase for Asymmetric C(sp³)-H Chlorination

40 results in Keywords: Directed Evolution