Organic Chemistry

A Spirobicyclo[3.1.0] Terpene from the Investigation of Sesquiter-pene Synthases from Lactarius deliciosus

Authors

  • Choon Boon Cheong Institute of Sustainability for Chemicals, Energy and Environment ,
  • Guangrong Peh Institute of Sustainability for Chemicals, Energy and Environment ,
  • Yifeng Wei Singapore Institute of Food and Biotechnology Innovation ,
  • Rehka T Singapore Institute of Food and Biotechnology Innovation ,
  • Ee Lui Ang Singapore Institute of Food and Biotechnology Innovation ,
  • Huimin Zhao Singapore Institute of Food and Biotechnology Innovation & University of Illinois at Urbana-Champaign ,
  • Congqiang Zhang Singapore Institute of Food and Biotechnology Innovation ,
  • Yee Hwee Lim Institute of Sustainability for Chemicals, Energy and Environment

Abstract

Milk cap mushrooms in the genus Lactarius are known to produce a wide variety of terpene natural products. However, their repertoire of terpene biosynthetic enzymes has not been fully explored. In this study, several candidate sesquiterpene synthases were identified from the genome of the saffron milk cap mushroom L. deliciosus, and expressed in a sesquiterpene-overproducing Escherichia coli strain. In addition to enzymes that produce several known terpenes, we identified an enzyme belonging to a previously unknown clade of sesquiterpene synthases that produces a terpene with a unique spirotricyclic scaffold. These findings further add to the rich diversity of terpene scaffolds and mushroom terpene synthases and are valuable for biotechnological applications in producing these terpenoids.

Content

Thumbnail image of Lacterius Spirobicyclo Terpene_preprint.pdf

Supplementary material

Thumbnail image of Lacterius Spirobicyclo Terpene SI.pdf
Supporting Information
SI containing methods and material of cell cultures, spectral characterization, additional figures, as well as GC-MS, HRMS, NMR, FTIR and elemental-analysis