Analytical Chemistry

Non-aqueous bonding of leuprorelin to Ochratoxin A for solid-phase extraction

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Abstract

The anticancer leuprorelin was found to have excellent affinity to ochratoxin A (OTA), with an equilibrium constant of 2.2 × 10^8 M^-1 at 273 K (dissociation constant Kd = 4.5 nM) when functionalized into a mesoporous polymer for binding to OTA. Binding between the surface-bound leuprorelin and mycotoxin was corroborated using DFT-based analysis, and it was extended to extraction of OTA from heavily fatty matrices of coffee, achieving 95% recovery with improved cyclability as compared with immunoaffinity.

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Experimental and Computational Details