Biological and Medicinal Chemistry

Investigation on the relationship between lipid composition and structure in model membranes composed of extracted natural phospholipids

Authors

Abstract

Unravelling the structural diversity of cellular membranes is a paramount challenge in life sciences. In particular, lipid composition affects the membrane collective behaviour, and its interactions with other biological molecules. Here, the relationship between membrane composition and resultant structural features was investigated by surface pressure-area isotherms, Brewster angle microscopy and neutron reflectometry on in vitro membrane models of the mammalian plasma and endoplasmic-reticulum-Golgi intermediate compartment membranes in the form of Langmuir monolayers. Natural extracted yeast lipids were used because, unlike synthetic lipids, the acyl chain saturation pattern of yeast and mammalian lipids are similar. The structure of the model membranes, orthogonal to the plane of the membrane, as well as their lateral packing, was found to depend strongly on their specific composition, with cholesterol having a major influence on the in-plane morphology, yielding a coexistence of liquid-order and liquid-disorder phases.

Content

Thumbnail image of Natural_ lipids_Santamaria_et_al.pdf

Supplementary material

Thumbnail image of SI_Natural_ lipids_Santamaria_et_al.pdf
Investigation on the relationship between lipid composition and out-of-plane structure in model membranes composed of extracted natural phospholipids
Supporting data from Neutron Reflectometry and Brewster Angle microscopy experiments.